shRNA Validation

In addition to the pooled CRISPR libraries we offer, there are a few other libraries that researchers can choose to use for gene knockout screens, such as the Broad Institute’s GeCKO and Brunello libraries available through Addgene. To conserve the limited stock of these libraries, many labs only distribute a...

The last blog entry discussed how hairpin structural features affect representation and effectiveness of shRNA sequences in pooled libraries. However, it is clear that the nucleotide sequence is possibly the major factor that determines the efficiency at which a particular siRNA or shRNA knocks down a...

We have done a significant amount of evaluation to identify features of the optimal shRNA structure necessary to ensure our complex libraries maintain and express representative and effective shRNA. Independent from particular targeting sequences, structural variations such as defined mismatches, loop...