Cellecta Scribe™ Vectors Enable RNA-Seq Analysis of Pooled CRISPR and RNAi Screens

October 04, 2017

Earlier this year, 3 separate studies (Adamson, et al., Datlinger, et al., and Dixit, et al.) demonstrated an approach to combine pooled CRISPR genetic screening with RNA expression profiling at the single-cell level. Integrating these two approaches enables knockout-specific expression data to be generated for...
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How to Make a Crappy CRISPR Library

June 01, 2017

In addition to the pooled CRISPR libraries we offer, there are a few other libraries that researchers can choose to use for gene knockout screens, such as the Broad Institute’s GeCKO and Brunello libraries available through Addgene. To conserve the limited stock of these libraries, many labs only distribute a...
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Improved sgRNA Design Delivers Better CRISPR Knockout Screen Results

April 07, 2016

Last year, Cellecta received a Phase 1 NIH SBIR grant to develop validated single guide RNA (sgRNA). The initial study was designed to investigate an approach to optimize CRISPR sgRNA to improve the efficiency of CRISPR knockout.The 5'-end of the CRISPR sgRNA contains 20 nucleotides specific to the gene targeted...
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Cellecta Gets SBIR Grant to Develop Validated CRISPR Library

July 22, 2015

Cellecta received a Phase I NIH SBIR Grant to develop CRISPR guide RNAs (gRNAs or sgRNAs) for human and mouse genes and then use these to build a knockout library targeting 6,500 key disease-related genes in major signal transduction pathways.Targeting a gene for knockout using CRISPR requires a short strand of...
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CRISPR Knockout Screens with Optimal Cas9 to sgRNA Ratios

March 12, 2015

The previous blog post " Two-Vector CRISPR System Is Better Approach for Knockout Screens" discussed the advantages of expressing the Cas9 nuclease on a vector separate from the one used for the guide RNAs (sgRNA)—a two-vector CRISPR system—when using the system for complex pooled loss-of-function screens.  In...
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