reproducibility

In addition to the pooled CRISPR libraries we offer, there are a few other libraries that researchers can choose to use for gene knockout screens, such as the Broad Institute’s GeCKO and Brunello libraries available through Addgene. To conserve the limited stock of these libraries, many labs only distribute a...

A couple of months ago at the CHI Discovery on Target Conference, Hakim Djaballah, Director of the HTS Core Facility at the Memorial Sloan Kettering Cancer Center, gave a unique and insightful presentation highlighting the challenges RNAi screening to identify lethal loss of function interaction in oncogenic...

Researchers are often interested in using a pooled shRNA library for genome-wide RNAi screening to cast a very “wide and unbiased net to identify any and all genes functionally involved in some pathway”. Although it is not difficult to make an shRNA library targeting all human or mouse genes, it is practically very...