Catalog #: CANA
Get results from gene screens with pooled CRISPR, shRNA, and barcode libraries faster by letting Cellecta do the NGS prep, sequencing, and analysis.
You provide Cellecta with frozen cell pellets, tissue or genomic DNA and we do the rest!
If you are running your own pooled lentiviral genetic screens with CRISPR sgRNA, RNAi hairpin, or using our cell barcode labeling libraries, simply provide harvested cells or genomic DNA for each time point or treatment condition (one sample), and we will provide you with a spreadsheet enumerating the read number for each of the library elements. Our Genetic Screens & Barcode Library Sequencing service...
Additional bioinformatics and pathway analyses including MAGeCK and others are available upon request.
Sequencing is provided not only for Cellecta libraries but also similar non-Cellecta CRISPR libraries, such as the GeCKO, Brunello, Brie libraries, etc. We have extensive experience in designing primers and running NGS on a large variety of pooled libraries for various types of genetic screens.
Alternatively, if you prefer to run NGS on the libraries yourself, Cellecta offers a variety of NGS Analysis kits with the primers you need to carry this out.