If you are running your own pooled lentiviral genetic screens with CRISPR sgRNA, RNAi hairpin, or using our cell barcode labeling libraries, simply provide harvested cells or genomic DNA for each time point or treatment condition (one sample), and we will provide you with a spreadsheet enumerating the read number for each of the library elements. Our Genetic Screens & Barcode Library Sequencing service...
- Extracts genomic DNA
- Amplifies barcodes or sgRNA
- Performs NGS on the Illumina NextSeq or HiSeq
- Enumerates shRNA, sgRNA, or barcode counts from raw sequencing data
Additional bioinformatics and pathway analyses including MAGeCK and others are available upon request.
Sequencing is provided not only for Cellecta libraries but also similar non-Cellecta CRISPR libraries, such as the GeCKO, Brunello, Brie libraries, etc. We have extensive experience in designing primers and running NGS on a large variety of pooled libraries for various types of genetic screens.
Alternatively, if you prefer to run NGS on the libraries yourself, Cellecta offers a variety of NGS Analysis kits with the primers you need to carry this out.