Catalog #: CRATEST
The CRISPRaTest™ Functional dCas9-Activator Assay Kit measures functional dCas9 transactivator (e.g., dCas-VPH) in any mammalian cell system. The CRISPRaTest Kit provides reagents to measure the activator strength of any Streptococcus pyogenes dCas9 transactivator (dCas9-TA) hybrid protein in cells designed to be used for CRISPRa experiments using a FACS-based assay. The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene that increases in expression when expressed in cells with active dCas9-TA. The kit provides enough reagents to measure this activity in 5-15 cell lines.