Webinar: Immunophenotyping of T-cell Receptor and B-cell Receptor Clonotypes

Date: Friday, September 17, 2021

Time: 11:00 am - 12:00 pm USA Eastern Time (ET); 08:00 am - 09:00 am USA Pacific Time (PT)

Speakers: Alex Chenchik, Ph.D.,President and Chief Scientific Officer, Cellecta, Inc.

 

Abstract:

T-cell receptor (TCR) and B-cell receptor (BCR) repertoire profiling holds great potential for understanding the disease mechanisms and development of new therapeutics for infectious disease, auto-immunity and immuno-oncology applications. However, this potential could be greatly improved by combining information about receptor clonotypes with immunophenotypes of T and B cells. To facilitate these studies, we developed a novel technology for combined profiling of all human TCR and BCR variable regions and phenotypic characterization of immune cells.

The developed TCR/BCR Immunophenotyping method involves multiplex RT-PCR amplification and sequencing of complementarity-determining regions (CDR) of TCR and BCR genes and a set of the most informative T- and B-cell phenotyping genes. Bioinformatics analysis of NGS data allows for the profiling of TCR/BCR clonotypes, as well as identifying major immune cell subtypes and their activation status.

Data will be presented showing how combined TCR/BCR clonotype analysis combined with targeted expression profiling of immune cells can be applied for large-scale discovery of novel cell typing and activation biomarkers in several immune-response model systems. Preliminary studies indicate the assay has unparalleled throughput, sensitivity and improved cost-effectiveness for high-throughput immunity biomarker discovery applications.

In this webinar, you will learn:

  • The importance of TCR and BCR repertoire profiling for understanding mechanisms of action
  • The value of combining information about receptor clonotypes with immunophenotypes of T and B cells
  • The potential application of this methodology for large-scale discovery of novel cell typing and activation biomarkers
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