The DriverMap Targeted Expression Profiling assay, a PCR-based approach, yields reliable data even from very low amounts of RNA. When used with mRNA retrieved by hybridization on an oligo-dT plate, simply wash and multiplex RT-PCR in the same wells. No additional transfers, precipitation, or centrifuging are required. The samples are transferred into a second index plate to prepare sequence-ready libraries.
More information on the DriverMap assay and workflow is available here.
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