DriverMap™ Cell-Free RNA & DNA Purification Kit

Cellecta's DriverMap™ Cell-Free RNA & DNA Purification Kit co-purifies cell-free RNA (cfRNA) and cell-free DNA (cfDNA) from human plasma in one prep. Its magnetic bead technology and buffers are optimized for high-yield recovery of degraded nucleic acids from extracellular vesicles (EVs), exosomes and the soluble plasma fraction.

The resulting purified cfNA is ready for these DriverMap™ cell-free assays:

1. DriverMap™ Cell-Free AIR TCR/BCR Profiling — CDR3 profiling of all 7 TCR/BCR chains from cfRNA + cfDNA.
2. DriverMap™ Cell-Free EXP 5K Profiling (available soon) — expression of ~5,000 human genes in plasma cfRNA, at least 100-fold more sensitive than RNA-seq.
3. DriverMap™ Cell-Free OncoVirus Detection (available soon) — identification of 26 cancer-associated viral sequences in cfRNA and cfDNA.

Key Benefits

1. One Prep, Two Biomarker Signals: Most cell-free workflows focus on cfDNA alone. The DriverMap™ Cell-Free Purification Kit keeps both fractions. cfRNA shows which cells are active, and cfDNA shows which cells are dying. You get both views from the same plasma sample, with no extra sample and no second extraction required.

2. Validated Workflow for Liquid-Biopsy Immune Receptor Profiling: The kit was designed to efficiently prepare samples for the cfAIR assay. It supplies the exact input the assay's two barcoded reactions need to separate lymphocyte activation (cfRNA) from lymphocyte turnover (cfDNA). This gives one validated workflow from blood draw to TCR/BCR clonotype detection.

3. Built to Maximize Data from Precious, Low-Input Plasma: Silane magnetic beads are optimized to capture short, degraded nucleic acids, which are present at only a few copies per mL of plasma. Spin-filter elution in as little as 40 µL gives a concentrated eluate ready for the sensitive, targeted multiplex PCR step. No vacuum manifold or automated extractor is needed.

Fig. 1. Estimation of purified cfRNA and cfDNA yields by GAPDH amplification. Purified cfRNA and cfDNA fractions were analyzed by RT-PCR and PCR, respectively, using primers targeting exon 2 of the GAPDH gene. Purification kits: 1, Cellecta DriverMap™ Cell-Free DNA & RNA Purification Kit; 2, MagMax cfNA kit; 3, Sera-Xtracta cfDNA kit; 4, Apostle cfNA kit.

Related Products

DriverMap™ Cell-Free AIR Profiling Kits

• Use with the DriverMap™ Cell-Free AIR TCR/BCR RNA & DNA Profiling Kit for liquid-biopsy immune repertoire profiling
• Use with the DriverMap™ Cell-Free EXP 5K and OncoVirus Detection kits (available soon)

Manuals

• User Manual: DriverMap™ Cell-Free RNA & DNA Purification Kit User Manual