Catalog #: SVRTC9E2B-PS
All-in-one Tetracycline-Inducible CRISPR Cas9 Lentiviral Vector. The tet-inducible Cas9 construct contains all the elements needed for transduction and stable integration of an inducible Cas9 gene for CRISPR-mediated gene editing.
A. The first option is for a construct expressing Cas9 when doxycycline is present in the cell medium. The Cas9 protein can be expressed for a limited period to catalyze genomic editing and then shut down for subsequent use of the engineered cells. This is available in plasmid or packaged formats.
B. The second option is a construct expressing Cas9-BFP fusion when doxycycline is present. This option allows real-time monitoring of induction by measuring BFP fluorescence. This is available in plasmid or packaged formats.
The vectors use the same optimized hybrid tet-regulated transactivator rtTA that forms the basis of Cellecta’s InDOXible Tet-Activated Lentiviral Expression System.
Cellecta also can make stable custom cell lines with robustly inducible and tightly regulated Cas9 expression for cell engineering and CRISPR screening applications.
|SVRTC9E2B-PS||Cas9 | Plasmid
|SVRTC9E2B-VS||Cas9 | Virus||pRTCE2Bla-TRE-Cas9-EFS-rtTA-2A-Blast|
|SVRTC9BfpE2B-PS||Cas9BFP fusion | Plasmid||pRTCBfpE2Bla-TRE-Cas9BFP-EFS-rtTA-2A-Blast|
|SVRTC9BfpE2B-VS||Cas9BFP fusion | Virus||pRTCBfpE2Bla-TRE-Cas9BFP-EFS-rtTA-2A-Blast|