Ready-to-Use Lentiviral Packaging Plasmid Mixes

Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mixes allow production of VSV-G pseudotyped viral particles when co-transfected into packaging cells together with second- or third-generation HIV-based lentiviral vectors.

The 2-Plasmid Lentiviral Packaging Mix (Catalog # CPCP-K2A) is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the structural, regulatory, and replication proteins needed to produce pseudotyped, packaged lentiviral expression constructs. 

For laboratories requiring a 3-Plasmid Lentiviral Packaging Mix (Catalog # CPCP-K3A), this kit contains 3 plasmids: the human immunodeficiency virus (HIV) lentiviral packaging plasmids pGAG and pREV, and the pMD2.G plasmid containing VSV-G. 

These plasmids provide all the necessary structural, regulatory, and replication proteins required to produce pseudotyped packaged lentiviral expression constructs when co-transfected with an HIV-based lentiviral expression construct (e.g., Cellecta pRSG sgRNA expression construct) into a producer 293T cell line (e.g., ATCC, Cat.# CRL-11268™). Pseudotyped lentiviral particles with VSV-G envelope protein can infect a variety of mammalian or non-mammalian, dividing or non-dividing cells.

The kits contain 250 µg of lentiviral packaging plasmid mix, sufficient for 25 transfections in 10-cm culture plates.

The individual packaging plasmids are available for purchase separately.

Manual and Product Documentation

• Manual: Lentiviral Construct Packaging and Transduction
• Safety Data Sheet (SDS): Packaging Mix SDS

Vector Information

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