Cellecta Blog & News

We have started the Cellecta blog as a simple, unobtrusive conduit that enables us to talk directly to our customers, collaborators, and other interested researchers about new and interesting developments related to Cellecta’s technology and business. Check back here often for the latest news and events.

About Cellecta

Cellecta Inc., a custom and contract functional genomics solutions provider, focuses primarily on developing and implementing flexible and scalable broad-based screening and analysis approaches for drug target and biomarker discovery. Our high throughput functional genetic screening portfolio includes shRNA and CRISPR screening services, custom and off-the-shelf pooled libraries, and knockout/knockdown cell lines that facilitate genome-wide functional screening and the identification and validation of genes involved in critical biological and pathological responses.

With our new DriverMap™ Gene Expression and Molecular Profiling Service, Cellecta now offers a quantitative, multiplexed approach that allows simultaneous profiling of thousands of genes in one reaction, thereby providing the transcriptome profile of the tumor microenvironment.

Screening for Synergistically Lethal Knockdown Combinations in Cancer Cells

August 31, 2011

Therapeutic approaches using multiple drug combinations have become a standard treatment model for many types of cancer. Due to the tremendous genetic complexity and adaptive nature of most human malignancies, the use of multiple drugs acting on different targets increases the efficacy and helps thwart the...

shRNA Design Results – How good is your algorithm?

August 03, 2011

DECIPHER Pooled shRNA Library shRNA shRNA Design shRNA Library shRNA Validation TechnicalDECIPHER

The last blog entry discussed how hairpin structural features affect representation and effectiveness of shRNA sequences in pooled libraries. However, it is clear that the nucleotide sequence is possibly the major factor that determines the efficiency at which a particular siRNA or shRNA knocks down a...

The Importance of shRNA Structural Design for Pooled Libraries

June 23, 2011

miRNA Pooled shRNA Library shRNA shRNA Design shRNA Validation TechnicalmiRNA

We have done a significant amount of evaluation to identify features of the optimal shRNA structure necessary to ensure our complex libraries maintain and express representative and effective shRNA. Independent from particular targeting sequences, structural variations such as defined mismatches, loop...

RNAi Screening with An Inducible Promoter: Is There an Advantage?

May 13, 2011

H1 in vivo inducible lethal Pooled shRNA Library RNAi Screen RNAi Screening shRNA Library Technicalapoptosis U6

Inducible expression is often desirable for functional genetic testing to establish clear cause and effect for a specific phenotype. A particular phenotype can be demonstrably linked to expression of a specific cDNA by showing it disappears when transcription is suppressed. Although less direct, similar logic...

Ensuring Comprehensive Screening with Pooled shRNA Expression Libraries

April 03, 2011

DECIPHER Pooled shRNA Library reproducibility RNAi Screen RNAi Screening shRNA shRNA Library TechnicalDECIPHER

Researchers are often interested in using a pooled shRNA library for genome-wide RNAi screening to cast a very “wide and unbiased net to identify any and all genes functionally involved in some pathway”. Although it is not difficult to make an shRNA library targeting all human or mouse genes, it is practically very...

SBS 2011 Schedule, March 30th

March 30, 2011

ConferencesFHCRC Fred Hutchinson Cancer Research Center RNAi Screen Roswell Park Cancer Institute RPCI SBS shRNA Library speaker The Scripps Research Institute TSRI tutorial

Take the opportunity at SBS 2011 to learn about Cellecta's cancer drug target discovery services. Come visit us at booth #522, and be sure not to miss our oral presentation and tutorial on Wednesday, March 30:HT RNAi Screening of Anti-Cancer Targets With Pooled shRNA LibrariesSpeaker: Alex Chenchik, Ph.D.Alex...

AACR-NCI Systems Biology Conference

March 12, 2011

Cancer conference Conferences Peptides poster RNAi Screen Softwarecancer

Cellecta attended the AACR-NCI Systems Biology : Confronting the Complexity of Cancer Conference in San Diego last week and presented two posters:...

The DECIPHER Project’s New Human Module Targets 5,000 More Genes

February 25, 2011

DECIPHER FHCRC Fred Hutchinson Cancer Research Center Pooled shRNA Library RNAi Screen RNAi ScreeningDECIPHER Roswell Park Cancer Institute RPCI shRNA Library The Scripps Research Institute TSRI

The addition of the 3rd DECIPHER Human shRNA Expression Library Module to the DECIPHER pooled shRNA library collection adds approximately 5,000 new cell surface, extracellular, and DNA binding target genes...

U6 or H1 - Which promoter is better?

February 18, 2011

lentiviral vector Lentiviral VectorsH1 neuronal cells promoter shRNA shRNA Library U6 vector

Since we provide both constitutive and tetracycline-inducible versions of both U6 and H1 shRNA promoters,...

Cellecta's DECIPHER Project RNAi Screening Tools at Roswell Park Cancer Institute

February 15, 2011

Cancer DECIPHER disease Pooled shRNA Library RNAi Screen RNAi Screening Roswell Park Cancer Institute RPCI shRNA Library signaling pathways TAM TAM Programcancer

Cellecta's recent agreement with the Roswell Park Cancer Institute (RPCI) provides support to laboratories in their institution doing genome-wide RNAi...

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