August 31, 2011
Therapeutic approaches using multiple drug combinations have become a standard treatment model for many types of cancer. Due to the tremendous genetic complexity and adaptive nature of most human malignancies, the use of multiple drugs acting on different targets increases the efficacy and helps thwart the...
Read More August 03, 2011
The last blog entry discussed how hairpin structural features affect representation and effectiveness of shRNA sequences in pooled libraries. However, it is clear that the nucleotide sequence is possibly the major factor that determines the efficiency at which a particular siRNA or shRNA knocks down a...
Read More June 23, 2011
We have done a significant amount of evaluation to identify features of the optimal shRNA structure necessary to ensure our complex libraries maintain and express representative and effective shRNA. Independent from particular targeting sequences, structural variations such as defined mismatches, loop...
Read More May 13, 2011
Inducible expression is often desirable for functional genetic testing to establish clear cause and effect for a specific phenotype. A particular phenotype can be demonstrably linked to expression of a specific cDNA by showing it disappears when transcription is suppressed. Although less direct, similar logic...
Read More April 03, 2011
Researchers are often interested in using a pooled shRNA library for genome-wide RNAi screening to cast a very “wide and unbiased net to identify any and all genes functionally involved in some pathway”. Although it is not difficult to make an shRNA library targeting all human or mouse genes, it is practically very...
Read More March 30, 2011
Take the opportunity at SBS 2011 to learn about Cellecta's cancer drug target discovery services. Come visit us at booth #522, and be sure not to miss our oral presentation and tutorial on Wednesday, March 30:HT RNAi Screening of Anti-Cancer Targets With Pooled shRNA LibrariesSpeaker: Alex Chenchik, Ph.D.Alex...
Read More March 12, 2011
Cellecta attended the AACR-NCI Systems Biology : Confronting the Complexity of Cancer Conference in San Diego last week and presented two posters:...
Read More February 25, 2011
The addition of the 3rd DECIPHER Human shRNA Expression Library Module to the DECIPHER pooled shRNA library collection adds approximately 5,000 new cell surface, extracellular, and DNA binding target genes...
Read More February 18, 2011
Since we provide both constitutive and tetracycline-inducible versions of both U6 and H1 shRNA promoters,...
Read More February 15, 2011
Cellecta's recent agreement with the Roswell Park Cancer Institute (RPCI) provides support to laboratories in their institution doing genome-wide RNAi...
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